Exosomal long non-coding RNA MALAT1: a candidate of liquid biopsy in monitoring of Wilms’ tumor
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In brief
In brief
This study identifies exosomal long non-coding RNA MALAT1 as a potential non-invasive biomarker for monitoring Wilms' tumor in pediatric patients. Researchers detected MALAT1 in exosomes from tissue culture, urine, and plasma samples, finding significantly downregulated expression in WT patients compared to controls.
Written by the GCMD Library team from the article.
Abstract
Purpose
Wilms’ tumor (WT) is a rare kidney cancer that primarily affects children. Exosomes are extracellular vesicles that cargo nucleic acids, proteins,etc. for cellular communication. Long non-coding RNAs (lncRNAs) have utility as biomarkers for cancer diagnosis, prognosis, and disease monitoring. We hypothesize that expression of lncRNA, metastasis-associated lung adenocarcinoma transcript-1(MALAT1), is dysregulated and possibly trafficked within exosomes to influence the tissue microenvironment for metastasis and recurrence of WT.
Methods
We investigated the expression of MALAT1 in thirty WT samples by qPCR. Exosomes were isolated using a precipitated and affinity-binding-based kit, and characterized using TEM, NTA, and DLS.
Results
Mean number of exosomes was 9.01×108/mL in primary culture, 1.64×108/mL in urine, and 4.65×108/plasma:400µl. Average yield of total RNA was 1.28µg (primary-culture supernatant:1ml), 1.47µg (Urine:1ml), 1.65µg (Plasma:400 µL). We quantified MALAT1 in exosomes derived from these sources in patients of WT. Expression of MALAT1 was significantly downregulated (p=0.008) in WT samples.
Conclusion
This is the first study that demonstrated the presence of lncRNA MALAT1 in various invasive and non-invasive samples of patients with WT(primary tissue culture, urine, and plasma samples).