CDH organoids derived after fetal display less differentially expressed genes compared to the one derived before the fetal procedure. So they are more similar to the control organoids.
Giussepe Cala, PhD candidate - Best of the Best in Pediatric Surgery 2024
▶Ep 29 · 0:20
clinicalAmniotic fluid is recirculated by different developing organs and contains stem cells.↗
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quotewe found out that CDH organoids exhibit a very different cell type composition when compared to healthy controls↗
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clinicalFetal organoids can be derived only after the termination of pregnancy when using traditional tissue-based methods.↗
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clinicalThe first amniotic fluid cell atlas revealed that the majority of cells present in the fluid are epithelial at the single cell level.↗
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clinicalEpithelial cells in amniotic fluid are shed from various developing organs including the gut, kidney, and lung.↗
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clinicalViable cells isolated from amniotic fluid can be cultured in 3D to form epithelial organoids of fetal origin that display different morphologies.↗
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clinicalGene expression profiling of amniotic fluid organoids shows they form three different groups: lung, kidney, and intestinal organoids.↗
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clinicalAmniotic and tracheal fluid can be collected from CDH fetuses undergoing tracheal occlusion.↗
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clinicalLung organoids can be expanded from the fluid of CDH patients and contain lung epithelial stem progenitor cells.↗
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clinicalCDH organoids derived after fetal tracheal occlusion display fewer differentially expressed genes compared to those derived before the procedure, making them more similar to control organoids.↗
▶Ep 29 · 0:20
opinionThe gene expression changes in CDH organoids indicate that fetal surgery is changing something at the molecular level, which is reflected in the organoid model.↗
▶Ep 29 · 0:20
clinicalCDH organoids appear to have impaired differentiation and function based on ciliary beating frequency measurements.↗
▶Ep 29 · 0:20
clinicalSingle-cell RNA sequencing revealed that CDH organoids exhibit a very different cell type composition when compared to healthy controls.↗
▶Ep 29 · 0:20
clinicalAmniotic fluid contains fetal epithelial stem cells that can form organoids in culture, and these organoids can be generated during pregnancy without using tissue samples.↗
▶Ep 29 · 0:20
clinicalLung organoids can be derived prenatally from the fluids of CDH fetuses.↗
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opinionThis organoid technology may serve in the future as a tool for functional prenatal diagnosis and clinical decision making.↗
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quotecan we generate fetal organoids without a fetal tissue sample? Can these organoids help prenatal diagnosis?↗
quotewe found that the majority of the cells present in the fluid are epithelial when observed at the single cell level↗
▶Ep 29 · 0:20
quoteCDH organoids derived after fetal display less differentially expressed genes compared to the one derived before the fetal procedure. So they are more similar to the control organoids.↗
▶Ep 29 · 0:20
quoteThis indicates that the fetal surgery is actually changing something, and this is reflected in our organoid model.↗
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quotewe observed that CDH organoids seem to have an impaired differentiation and also function↗
▶Ep 29 · 0:20
clinicalOrganoids are an advanced in vitro system grown from tissue-specific stem cells that can be derived from fetal or adult tissues and used to study tissue biology and diseases.↗
▶Ep 29 · 4:56
opinionFetal organoids can be used to study development because it is difficult to access fetal tissue, providing a different tool to study the epithelium of fetuses.↗
▶Ep 29 · 4:56
clinicalOrganoids can be used to test both known compounds already working in clinics and new compounds, with the outcome of organoid phenotype after treatment correlated with patient clinical outcomes.↗
▶Ep 29 · 4:56
opinionFor the CDH organoid system, the translational potential is in drug testing rather than transplantation.↗
▶Ep 29 · 6:22
clinicalA drug testing platform is being established to test as many drugs as possible on CDH organoids, with interest in drugs that enhance proliferation of epithelial cells.↗
▶Ep 29 · 6:22
clinicalIn CDH, the lungs are hypoplastic, and drugs could potentially improve lung maturation by testing on organoids.↗
▶Ep 29 · 6:22
opinionDrugs tested on organoids could potentially be implemented with the endoluminal tracheal occlusion procedure, similar to how nanoparticles could be implemented.↗
▶Ep 29 · 7:13
clinicalA patent is ongoing for the organoid technology but the organoids are not yet commercially available.↗
