Combined application of Indocyanine green (ICG) and laser lead to targeted tumor cell destruction
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Read the article on jpedsurg.org ↗Article · Sep 2018 · 1 min read
In brief
In brief
Preclinical study demonstrates that pulsed laser treatment combined with indocyanine green (ICG) injection achieves selective neuroblastoma cell death in decapsulated tumors without damaging surrounding healthy tissues. This targeted photothermal approach may offer a precision tool for challenging pediatric solid tumors adherent to vital structures.
Written by the GCMD Library team from the article.
Purpose
Precise excision of neuroblastoma is challenging, especially when tumors adhere to vital structures. Indocyanine green (ICG), an FDA-approved dye with absorption peaking at 800 nm, can absorb the near IR laser energy and release heat in the dyed tissue. We hypothesize that by injecting ICG at tumor sites followed by precise laser application, tumor cell death can be selectively targeted.
Methods
Orthotopic neuroblastoma tumors were created in the adrenal gland of immunocompromised mice. Tumor, liver, kidney, and muscle tissues were chosen for ICG injection. Intervention variables included presence of tumor capsule, continuous vs. pulsed laser treatment and total energy delivered. Control groups included laser or ICG only. Tissues were stained with hematoxylin/eosin.
Results
Continuous wave laser generated excessive heat, causing damage in all tissues. When using pulsed laser treatment, liver, kidney, muscle, and intact tumor tissues showed no cell death when treated with laser alone or laser plus ICG. Tumor tissue with the capsule removed, however, showed cell death on histology.
Conclusions
Pulsed laser treatment combined with ICG causes targeted tumor cell death in neuroblastoma tumor without capsule. No cell death was observed when tumor capsule was present, when only laser was used, or when applied over non-tumor tissues.